Tamachkiarow L, Flemming H C
Institute for Interfacial Biotechnology, University of Duisburg, Geibelstr. 41, D-47057 Duisburg, Germany.
Water Sci Technol. 2003;47(5):19-24.
Any advanced anti-fouling strategy must be based on early warning systems which allow for timely, precisely directed and optimized countermeasures. Such systems must be able to detect biofilm growth on representative surfaces. In order to meet this requirement, a fibre optical device (FOS) has been developed. It is based on light which is scattered by objects deposited on the tip of an optical fibre. A receiving fibre collects the signal and forwards it to a detection and quantification unit. Both the sending and the receiving fibre are mounted in a measuring head which is integrated evenly on the inner surface of a water pipeline at representative sites. This device was applied to a water system of a brewery in order toput its reliability to test under practical conditions. The FOS detected the build-up of a deposit which was identified independently as consisting of microorganisms, i.e., a biofilm. A stable, well detectable and reproducible signal could be obtained above a colonization of 10(5) cells cm-2. Adjustment of the sensitivity of the amplifier allowed for detection of biofilms up to 10(10) cells cm-2. Cleaning countermeasures could be detected clearly by a decrease of backscattered light intensity. The system proved to be suitable for on-line, non-destructive, real-time and automatic monitoring for a period of almost two years, and thus, provides an important constituent for an advanced anti-fouling strategy.
任何先进的防污策略都必须基于预警系统，该系统能够实现及时、精准定向且优化的应对措施。此类系统必须能够检测代表性表面上生物膜的生长情况。为满足这一要求，已研发出一种光纤装置（FOS）。它基于沉积在光纤尖端的物体对光的散射原理。一根接收光纤收集信号并将其转发至检测和量化单元。发送光纤和接收光纤均安装在一个测量头中，该测量头均匀地集成在输水管道内表面的代表性位置。该装置应用于一家啤酒厂的水系统，以便在实际条件下测试其可靠性。FOS检测到一种沉积物的形成，经独立鉴定其由微生物组成，即生物膜。在细胞定植密度达到10⁵个/cm²以上时，可获得稳定、易于检测且可重复的信号。通过调节放大器的灵敏度，能够检测到细胞定植密度高达10¹⁰个/cm²的生物膜。通过背向散射光强度的降低，可以清晰地检测到清洁应对措施。该系统在近两年的时间里被证明适用于在线、无损、实时和自动监测，因此，为先进的防污策略提供了一个重要组成部分。
