Noda N, Ebie Y, Matsumura M, Tsuneda S, Hirata A, Inamori Y
National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8666, Japan.
Water Sci Technol. 2003;47(5):129-32.
The in situ fluorescent antibody and fluorescence in situ hybridization (FISH) methods are very useful in the in situ detection of specific bacteria like nitrifiers in a biofilm. In this study, simultaneous staining using the FISH and in situ fluorescent antibody methods was examined. As a result, no specific fluorescence was observed with either method when FISH was performed followed by the in situ fluorescent antibody method; however, when the in situ fluorescent antibody method was performed first followed by FISH, specific fluorescence was observed in both cases. Moreover, it was suggested that the detection specificities of FISH and the in situ fluorescent antibody method are almost identical.
原位荧光抗体法和荧光原位杂交（FISH）方法在生物膜中对特定细菌（如硝化细菌）的原位检测中非常有用。在本研究中，对使用FISH和原位荧光抗体法进行同步染色进行了检测。结果，当先进行FISH然后进行原位荧光抗体法时，两种方法均未观察到特异性荧光；然而，当先进行原位荧光抗体法然后进行FISH时，两种情况下均观察到了特异性荧光。此外，表明FISH和原位荧光抗体法的检测特异性几乎相同。
