Keinänen M M, Martikainen P J, Korhonen L K, Suutari M H
Department of Environmental Health, National Public Health Institute, P.O. Box 95, FIN-70701 Kuopio, Finland.
Water Sci Technol. 2003;47(5):143-7.
The development of microbial communities in biofilms of a drinking water distribution system was monitored, and compared to the microbial communities in water. The microbial communities were studied by phospholipid fatty acid (PLFA) profiles. In drinking water samples the most common PLFAs, with the proportion of 60.9%, were monoenoic fatty acids, such as 16:1omega7c and 18:1omega7c, indicating high abundance of gram-negative bacteria. Instead, in biofilm samples saturated fatty acids, such as 16:0 and 18:0, indicating general biomass, accounted for 54.9-78.4% of the total PLFAs. The proportions of monoenoic fatty acids in biofilm increased from 11.5% to 31.2% with water aging from 22 h to 62 h in the distribution system. In conclusion, water aging affected the structure of microbial communities in biofilms, and the microbes in water differed from those in biofilms. These differences might also reflect the differences in the physiological state of the microbes, which is influenced by water chemistry and by the growth environment, i.e. water or biofilm.
对饮用水分配系统生物膜中微生物群落的发展进行了监测，并与水中的微生物群落进行了比较。通过磷脂脂肪酸（PLFA）谱研究微生物群落。在饮用水样本中，最常见的PLFA（占比60.9%）是单烯脂肪酸，如16:1ω7c和18:1ω7c，表明革兰氏阴性菌数量众多。相反，在生物膜样本中，表明总体生物量的饱和脂肪酸，如16:0和18:0，占总PLFA的54.9 - 78.4%。在分配系统中，随着水龄从22小时增加到62小时，生物膜中单烯脂肪酸的比例从11.5%增加到31.2%。总之，水龄影响了生物膜中微生物群落的结构，水中的微生物与生物膜中的微生物不同。这些差异也可能反映了微生物生理状态的差异，而微生物生理状态受水化学和生长环境（即水或生物膜）的影响。
