β-糖苷酶与糖尿病微血管病变。II. β-N-乙酰氨基葡萄糖苷酶的一种胰岛素依赖同工酶。

Beta-glycosidases and diabetic microangiopathy. II. An insulin-dependent isozyme of beta-N-acetylglucosaminidase.

作者信息

Fushimi H, Tarui S

出版信息

J Biochem. 1976 Feb;79(2):271-5. doi: 10.1093/oxfordjournals.jbchem.a131067.

DOI:10.1093/oxfordjournals.jbchem.a131067

Abstract

Previously we reported that beta-glycosidase activities were markedly decreased in the kidney but increased in the serum of diabetic rats. To examine these changes, the isozymes of beta-N-acetylglucosaminidase [EC 3.2.1.30] of rats were examined by DEAE-cellulose column chromatography. At least 3 major isozymes were found in both the kidney and liver. The main isozyme was type II isozyme in normal rat kidney and type III in normal rat liver. The activity of the type II isozyme in the kidney was markedly lowered when the total activity was decreased in diabetes and its normal activity was restored on insulin treatment, in parallel with increase in the total activity in diabetes. No significant change was found in the chromatographic pattern of isozymes in the liver in diabetes. In diabetic rat serum, the increase of total activity was found to be due to increase of type I and II isozymes.

摘要

此前我们报道，糖尿病大鼠肾脏中的β-糖苷酶活性显著降低，而血清中的活性升高。为研究这些变化，通过DEAE-纤维素柱色谱法检测了大鼠β-N-乙酰氨基葡萄糖苷酶[EC 3.2.1.30]的同工酶。在肾脏和肝脏中均发现至少3种主要同工酶。正常大鼠肾脏中的主要同工酶为II型同工酶，正常大鼠肝脏中的主要同工酶为III型同工酶。糖尿病时总活性降低，肾脏中II型同工酶的活性显著降低，胰岛素治疗后其正常活性恢复，同时糖尿病时总活性增加。糖尿病时肝脏中同工酶的色谱模式未发现明显变化。在糖尿病大鼠血清中，总活性的增加是由于I型和II型同工酶的增加。