Woodward L A, Arimitsu H, Hirst R, Oguma K
Department of Microbiology and Immunology, James Cook University, Townsville 4811, Australia.
Infect Immun. 2003 May;71(5):2941-4. doi: 10.1128/IAI.71.5.2941-2944.2003.
Two proteins representing the heavy-chain subunits of botulinum neurotoxin types C and D were expressed in Escherichia coli, and their vaccine potential was evaluated. Mice were vaccinated with doses ranging from 0.5 to 10 microg and were challenged with 10 to 10(5) 50% lethal doses of toxin. For the type C subunit protein, C50, two doses of 2 microg were required for full protection, while, for type D subunit protein, D50, two 1- microg doses were required. A bivalent vaccine consisting of a mixture of these two proteins also provided protection against both botulinum neurotoxin type C and type D challenge. Antibody levels in serum were determined by both enzyme-linked immunosorbent assays and serum neutralization assays
在大肠杆菌中表达了代表肉毒杆菌神经毒素C型和D型重链亚基的两种蛋白质，并评估了它们作为疫苗的潜力。用0.5至10微克的剂量对小鼠进行免疫接种，然后用10至10(5)个50%致死剂量的毒素进行攻击。对于C型亚基蛋白C50，需要两剂2微克才能提供完全保护，而对于D型亚基蛋白D50，则需要两剂1微克。由这两种蛋白质混合物组成的二价疫苗也能提供针对肉毒杆菌神经毒素C型和D型攻击的保护。通过酶联免疫吸附测定和血清中和测定来确定血清中的抗体水平。
