Kimura K, Fujii N, Tsuzuki K, Murakami T, Indoh T, Yokosawa N, Takeshi K, Syuto B, Oguma K
Department of Epidemiology, Hokkaido Institute of Public Health, Hokkaido University, Japan.
Biochem Biophys Res Commun. 1990 Sep 28;171(3):1304-11. doi: 10.1016/0006-291x(90)90828-b.
Two DNA fragments, 3 kbp and 7.8kbp, which encode the type C1 botulinum neurotoxin gene, were obtained from toxigenic bacteriophage DNA by treatment with a restriction enzyme. They were cloned into the plasmid vectors for nucleotide sequence determination. The nucleotide sequence contained a single open reading frame coding for 1,291 amino acids corresponding to a polypeptide with a molecular weight of 149,000. The amino acid sequence of the C1 toxin has a few regions highly homologous with tetanus toxin.
