Shewen Patricia E, Lee Chiang W, Perets Ann, Hodgins Douglas C, Baldwin Katherine, Lo Reggie Y C
Department of Pathobiology, University of Guelph, Guelph, Ont., Canada N1G 2W1.
Vaccine. 2003 May 16;21(17-18):1901-6. doi: 10.1016/s0264-410x(03)00002-1.
Secreted recombinant sialoglycoprotease fusion protein (Gcp-F) of Mannheimia (Pasteurella) haemolytica A1 was examined for its ability to protect cattle from experimental challenge with M. haemolytica A1. Five M. haemolytica vaccines were compared including Gcp-F, logarithmic phase culture supernate (Presponse) and Presponse enriched with Gcp-F, recombinant leukotoxin (rLkt) or both. All calves receiving Gcp-F had significant serum antibody responses to this antigen, measured by ELISA, prior to challenge. Those vaccinated with Gcp-F alone had significantly lower percent pneumonic tissue than unvaccinated controls and a trend (P=0.085, one-tailed test) to lower clinical scores. Calves receiving Presponse with Gcp-F and rLkt had lower percent pneumonic tissue than those receiving Presponse alone, and calves receiving Presponse enriched with Gcp-F and/or rLkt had lower mean clinical scores, but the differences were not significant. This trial demonstrates the protective capacity of sialoglycoprotease. While, remarkably, recombinant Gcp-F provided some protection alone the results support its practical potential as a component of a multiple antigen vaccine.
对溶血曼氏杆菌（巴斯德氏菌属）A1分泌的重组唾液酸糖蛋白酶融合蛋白（Gcp-F）保护牛免受溶血曼氏杆菌A1实验性攻击的能力进行了检测。比较了五种溶血曼氏杆菌疫苗，包括Gcp-F、对数期培养物上清液（预反应液）以及富含Gcp-F的预反应液、重组白细胞毒素（rLkt）或二者兼具的疫苗。在攻击前，通过酶联免疫吸附测定法（ELISA）检测发现，所有接受Gcp-F的犊牛对该抗原有显著的血清抗体反应。单独接种Gcp-F的犊牛，其肺部组织病变百分比显著低于未接种的对照犊牛，且临床评分有降低趋势（单尾检验，P = 0.085）。接受含Gcp-F和rLkt的预反应液的犊牛，其肺部组织病变百分比低于仅接受预反应液的犊牛；接受富含Gcp-F和/或rLkt的预反应液的犊牛，其平均临床评分较低，但差异不显著。该试验证明了唾液酸糖蛋白酶的保护能力。虽然值得注意的是，重组Gcp-F单独提供了一定保护，但结果支持其作为多抗原疫苗成分的实际潜力。
