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Immunization with recombinant PlpE of ovine isolate provides protection against lethal challenge in mice.用绵羊分离株的重组PlpE进行免疫可使小鼠免受致死性攻击。
Iran J Vet Res. 2021 Fall;22(4):272-276. doi: 10.22099/ijvr.2021.37923.5522.
2
Characterization of immunodominant and potentially protective epitopes of Mannheimia haemolytica serotype 1 outer membrane lipoprotein PlpE.溶血曼氏杆菌1型外膜脂蛋白PlpE的免疫显性和潜在保护性表位的鉴定
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Immunity of cattle following vaccination with a Mannheimia haemolytica chimeric PlpE-LKT (SAC89) protein.用溶血曼氏杆菌嵌合PlpE-LKT（SAC89）蛋白对牛进行疫苗接种后的免疫情况。
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本文引用的文献

1
Cloning and Expression of Mannheimia haemolytica PlpE Gene in Escherichia coli and its Immunogenicity Assessment.溶血曼氏杆菌PlpE基因在大肠杆菌中的克隆、表达及其免疫原性评估
Arch Razi Inst. 2019 Jun;74(2):111-118. doi: 10.22092/ari.2018.116479.1169. Epub 2019 Jun 1.
2
Mannheimia haemolytica in bovine respiratory disease: immunogens, potential immunogens, and vaccines.牛呼吸道疾病中的溶血曼氏杆菌：免疫原、潜在免疫原及疫苗
Anim Health Res Rev. 2018 Dec;19(2):79-99. doi: 10.1017/S1466252318000142.
3
A chimeric protein comprising the immunogenic domains of Mannheimia haemolytica leukotoxin and outer membrane protein PlpE induces antibodies against leukotoxin and PlpE.一种包含溶血曼氏杆菌白细胞毒素免疫原性结构域和外膜蛋白PlpE的嵌合蛋白可诱导产生针对白细胞毒素和PlpE的抗体。
Vet Immunol Immunopathol. 2016 Jul;175:36-41. doi: 10.1016/j.vetimm.2016.05.004. Epub 2016 May 9.
4
LKTA and PlpE small fragments fusion protein protect against Mannheimia haemolytica challenge.LKTA 和 PlpE 小片段融合蛋白可抵抗溶血曼海姆菌的侵袭。
Res Vet Sci. 2012 Dec;93(3):1293-300. doi: 10.1016/j.rvsc.2012.07.004. Epub 2012 Jul 26.
5
Immunity of cattle following vaccination with a Mannheimia haemolytica chimeric PlpE-LKT (SAC89) protein.用溶血曼氏杆菌嵌合PlpE-LKT（SAC89）蛋白对牛进行疫苗接种后的免疫情况。
Vaccine. 2009 Mar 10;27(11):1771-6. doi: 10.1016/j.vaccine.2008.09.028. Epub 2008 Oct 1.
6
Mannheimia haemolytica chimeric protein vaccine composed of the major surface-exposed epitope of outer membrane lipoprotein PlpE and the neutralizing epitope of leukotoxin.由外膜脂蛋白PlpE的主要表面暴露表位和白细胞毒素的中和表位组成的溶血曼氏杆菌嵌合蛋白疫苗。
Vaccine. 2008 Sep 8;26(38):4955-61. doi: 10.1016/j.vaccine.2008.07.023. Epub 2008 Jul 31.
7
A multidomain outer membrane protein from Pasteurella multocida: modelling and simulation studies of PmOmpA.多杀性巴氏杆菌的一种多结构域外膜蛋白：PmOmpA的建模与模拟研究
Biochim Biophys Acta. 2007 Nov;1768(11):2831-40. doi: 10.1016/j.bbamem.2007.07.025. Epub 2007 Aug 16.
8
Recombinant Mannheimia haemolytica serotype 1 outer membrane protein PlpE enhances commercial M. haemolytica vaccine-induced resistance against serotype 6 challenge.重组溶血曼氏杆菌1型外膜蛋白PlpE增强了商业化溶血曼氏杆菌疫苗诱导的对6型攻击的抵抗力。
Vaccine. 2006 Mar 20;24(13):2248-55. doi: 10.1016/j.vaccine.2005.11.036. Epub 2005 Dec 9.
9
Immunogenicity of recombinant Mannheimia haemolytica serotype 1 outer membrane protein PlpE and augmentation of a commercial vaccine.重组溶血曼氏杆菌1型外膜蛋白PlpE的免疫原性及一种商业疫苗的增强作用。
Vaccine. 2003 Jun 20;21(21-22):2821-9. doi: 10.1016/s0264-410x(03)00213-5.
10
Efficacy of recombinant sialoglycoprotease in protection of cattle against pneumonic challenge with Mannheimia (Pasteurella) haemolytica A1.重组唾液酸糖蛋白酶对牛抵御溶血曼氏杆菌（溶血巴斯德氏菌）A1所致肺部感染的保护作用。
Vaccine. 2003 May 16;21(17-18):1901-6. doi: 10.1016/s0264-410x(03)00002-1.

用绵羊分离株的重组PlpE进行免疫可使小鼠免受致死性攻击。

Immunization with recombinant PlpE of ovine isolate provides protection against lethal challenge in mice.

作者信息

Yektaseresht A, Hemati Z, Sabet Sarvestani F, Hosseini A, Vir Singh S

机构信息

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.

出版信息

Iran J Vet Res. 2021 Fall;22(4):272-276. doi: 10.22099/ijvr.2021.37923.5522.

DOI:10.22099/ijvr.2021.37923.5522
PMID:35126534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8806166/
Abstract

BACKGROUND

primarily causes pneumonia leading to heavy morbidity and mortality in domestic livestock world-wide. Recently, outer membrane lipoproteins have emerged as targets for inducing protective immunity against the infection.

AIMS

This study aimed to evaluate recombinant outer membrane lipoprotein E (PlpE) from the ovine isolate, as a potential vaccine candidate

METHODS

Recombinant PlpE was constructed using pET26 (b) expression vector in . Expressed recombinant PlpE was purified and injected subcutaneously to mice. The protection index of the vaccine was evaluated by challenge of mice intraperitoneally.

RESULTS

Anti-PlpE antibody responses in the immunized mice was significantly increased in comparison with the control group which in turn, provided effective protection when challenged with strain of virulent .

CONCLUSION

Recombinant PlpE from ovine isolate had the potential to be used as a vaccine candidate against infection in sheep flocks.

摘要

背景

主要导致全球范围内家畜发生肺炎，造成严重的发病率和死亡率。最近，外膜脂蛋白已成为诱导针对该感染的保护性免疫的靶点。

目的

本研究旨在评估来自绵羊分离株的重组外膜脂蛋白E（PlpE）作为潜在的疫苗候选物。

方法

使用pET26（b）表达载体在大肠杆菌中构建重组PlpE。表达的重组PlpE经纯化后皮下注射到小鼠体内。通过对小鼠进行腹腔攻击来评估疫苗的保护指数。

结果

与对照组相比，免疫小鼠中抗PlpE抗体反应显著增加，当用强毒株攻击时，该抗体反应反过来提供了有效的保护。

结论

来自绵羊分离株的重组PlpE有潜力用作预防绵羊群感染的疫苗候选物。