Yektaseresht A, Hemati Z, Sabet Sarvestani F, Hosseini A, Vir Singh S
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.
Iran J Vet Res. 2021 Fall;22(4):272-276. doi: 10.22099/ijvr.2021.37923.5522.
primarily causes pneumonia leading to heavy morbidity and mortality in domestic livestock world-wide. Recently, outer membrane lipoproteins have emerged as targets for inducing protective immunity against the infection.
This study aimed to evaluate recombinant outer membrane lipoprotein E (PlpE) from the ovine isolate, as a potential vaccine candidate
Recombinant PlpE was constructed using pET26 (b) expression vector in . Expressed recombinant PlpE was purified and injected subcutaneously to mice. The protection index of the vaccine was evaluated by challenge of mice intraperitoneally.
Anti-PlpE antibody responses in the immunized mice was significantly increased in comparison with the control group which in turn, provided effective protection when challenged with strain of virulent .
Recombinant PlpE from ovine isolate had the potential to be used as a vaccine candidate against infection in sheep flocks.
主要导致全球范围内家畜发生肺炎，造成严重的发病率和死亡率。最近，外膜脂蛋白已成为诱导针对该感染的保护性免疫的靶点。
本研究旨在评估来自绵羊分离株的重组外膜脂蛋白E（PlpE）作为潜在的疫苗候选物。
使用pET26（b）表达载体在大肠杆菌中构建重组PlpE。表达的重组PlpE经纯化后皮下注射到小鼠体内。通过对小鼠进行腹腔攻击来评估疫苗的保护指数。
与对照组相比，免疫小鼠中抗PlpE抗体反应显著增加，当用强毒株攻击时，该抗体反应反过来提供了有效的保护。
来自绵羊分离株的重组PlpE有潜力用作预防绵羊群感染的疫苗候选物。
