Elgjo K, Hennings H, Michael D, Yuspa S H
J Invest Dermatol. 1976 May;66(5):292-6. doi: 10.1111/1523-1747.ep12482235.
Epidermal cells were separated from newborn mouse skin and grown on glass chamber slides or in plastic Petri dishes. Cell proliferation was examined at short intervals during the first 3 to 4 days in culture. DNA synthesis was estimated by the incorporation of [3H]thymidine into epidermal DNA, or by the labeling index. The mitotic rate was estimated by blocking mitotic cells with vinblastine. Reasonable agreement was found with the different methods. The cells grew synchronously with peaks of DNA synthesis at 21 to 23 hr, 35 to 40 hr, and 60 hr; peak mitotic rates were seen at 44 to 48 hr and 72 to 76 hr. The cells appeared to grow exponentially during the first 3 to 4 days in culture, with one main cohort of cells dividing during the successive proliferative peaks.
从新生小鼠皮肤中分离出表皮细胞，并将其培养在玻璃腔室载玻片上或塑料培养皿中。在培养的前3至4天内每隔一段时间检查细胞增殖情况。通过将[3H]胸腺嘧啶核苷掺入表皮DNA或通过标记指数来估计DNA合成。通过用长春花碱阻断有丝分裂细胞来估计有丝分裂率。发现不同方法之间有合理的一致性。细胞同步生长，DNA合成高峰出现在21至23小时、35至40小时和60小时；有丝分裂率高峰出现在44至48小时和72至76小时。在培养的前3至4天内，细胞似乎呈指数生长，在连续的增殖高峰期间有一个主要的细胞群体进行分裂。
