Pollack S, Lasky F D
J Lab Clin Med. 1976 Apr;87(4):670-9.
A new iron-binding protein contained in guinea pig intestinal mucosa has been purified to homogeneity. The protein has a molecular weight of 78,000 in a nondissociating system and 43,000 in SDS-gel electrophoresis. It binds approximately 2 moles of iron per mole with a formation constant of 10(19) at pH 7. It is distinguished from transferrin and lactoferrin by differences on DEAE-Sephadex chromatography and spectroscopy and by its failure to cross-react with antisera to these other iron-binding proteins.
豚鼠肠黏膜中含有的一种新的铁结合蛋白已被纯化至同质。该蛋白在非解离系统中的分子量为78,000，在SDS-凝胶电泳中的分子量为43,000。它在pH 7时每摩尔结合约2摩尔铁，形成常数为10(19)。通过在DEAE-葡聚糖凝胶柱色谱和光谱上的差异以及它不能与针对这些其他铁结合蛋白的抗血清发生交叉反应，可将其与转铁蛋白和乳铁蛋白区分开来。
