Thamer G, Kommerell B
J Lab Clin Med. 1976 Apr;87(4):734-8.
A radioimmunologic test system for determining the subtypes D and Y of the hepatitis-B surface antigen (HBs-Ag, Australia antigen, HAA) is described. This technique uses the immunoadsorption principle. The antigen to be subtyped is incubated with an antiserum which contains (125)I-labeled antibodies to the antigenic determinants a, d, and y. The specificity of the antibody which is not consumed in forming the antigen-antibody complex is determined by absorption on agarose to which HBs-Ag of subtypes D and Y is coupled. This specificity permits conclusions as to the subtype of the hepatitis-B surface antigen which is being investigated.
本文描述了一种用于测定乙型肝炎表面抗原（HBs-Ag，澳大利亚抗原，HAA）D和Y亚型的放射免疫检测系统。该技术采用免疫吸附原理。将待分型的抗原与一种抗血清一起孵育，该抗血清含有针对抗原决定簇a、d和y的（125）I标记抗体。未在形成抗原-抗体复合物过程中被消耗的抗体的特异性，通过在琼脂糖上的吸附来确定，该琼脂糖偶联了D和Y亚型的HBs-Ag。这种特异性使得能够对所研究的乙型肝炎表面抗原的亚型得出结论。
