Donea-Debroise B, Brocteur J, Andre A, Remacle M B
Biomedicine. 1979 Nov;30(5):260-4.
A radioimmunoassay determination of the HBs antigen subtypes is discussed, it is simple but effective technique was used in association with the use of the Austria II kit (Abbott Laboratories). This method consists of an inhibition reaction of the Austria II test, by previous incubation of the antigen to be subtyped with a monospecific antibody. With this method we were able to distinguish the y and the d antigens as well as the w1, w3, w4 determinants of hepatitis B surface antigen. We have included a frequency table of the various HBs subtypes found among donor and patient populations in Liège.
本文讨论了用放射免疫分析法测定乙肝表面抗原亚型，采用了一种简单但有效的技术，与奥地利II试剂盒（雅培实验室）联合使用。该方法通过将待分型的抗原与单特异性抗体预先孵育，来抑制奥地利II试验的反应。用这种方法，我们能够区分乙肝表面抗原的y和d抗原以及w1、w3、w4决定簇。我们还列出了在列日的献血者和患者群体中发现的各种乙肝表面抗原亚型的频率表。
