Smith Richard W P, Nasheuer Heinz-Peter
Abteilung Biochemie, Institut für Molekulare Biotechnologie, Jena, Germany.
Eur J Biochem. 2003 May;270(9):2030-7. doi: 10.1046/j.1432-1033.2003.03579.x.
Host species specificity of the polyomaviruses simian virus 40 (SV40) and mouse polyomavirus (PyV) has been shown to be determined by the host DNA polymerase alpha-primase complex involved in the initiation of both viral and host DNA replication. Here we demonstrate that DNA replication of the related human pathogenic polyomavirus JC virus (JCV) can be supported in vitro by DNA polymerase alpha-primase of either human or murine origin indicating that the mechanism of its strict species specificity differs from that of SV40 and PyV. Our results indicate that this may be due to differences in the interaction of JCV and SV40 large T antigens with the DNA replication initiation complex.
多瘤病毒猿猴病毒40（SV40）和小鼠多瘤病毒（PyV）的宿主物种特异性已被证明是由参与病毒和宿主DNA复制起始的宿主DNA聚合酶α-引物酶复合物决定的。在这里，我们证明，相关的人类致病性多瘤病毒JC病毒（JCV）的DNA复制在体外可以由人源或鼠源的DNA聚合酶α-引物酶支持，这表明其严格物种特异性的机制不同于SV40和PyV。我们的结果表明，这可能是由于JCV和SV40大T抗原与DNA复制起始复合物相互作用的差异所致。
