引发酶亚基p48的不同区域在体外控制小鼠多瘤病毒和猿猴病毒40的DNA复制。 - Suppr | 超能文献

引发酶亚基p48的不同区域在体外控制小鼠多瘤病毒和猿猴病毒40的DNA复制。

Different regions of primase subunit p48 control mouse polyomavirus and simian virus 40 DNA replication in vitro.

作者信息

Kautz A R, Schneider A, Weisshart K, Geiger C, Nasheuer H P

机构信息

Abteilung Biochemie, Institut für Molekulare Biotechnologie e.V., D-07745 Jena, Germany.

出版信息

J Virol. 2001 Feb;75(4):1751-60. doi: 10.1128/JVI.75.4.1751-1760.2001.

DOI:10.1128/JVI.75.4.1751-1760.2001

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC114084/

Abstract

DNA polymerase alpha-primase (pol-prim), a complex consisting of four subunits, is the major species-specific factor for mouse polyomavirus (PyV) and simian virus 40 (SV40) DNA replication. Although p48 is the most conserved subunit of pol-prim, it is required for in vitro PyV DNA replication but can inhibit cell-free SV40 DNA replication. Production of chimeric human-mouse p48 revealed that different regions of p48 are involved in supporting PyV DNA replication and inhibiting SV40 DNA replication. The N and C-terminal parts of p48 do not have species-specific functions in cell-free PyV DNA replication, but the central part (amino acids [aa] 129 to 320) controls PyV DNA replication in vitro. However, PyV T antigen physically binds to mouse, human, and chimeric pol-prim complexes independently, whether they support PyV DNA replication or not. In contrast to the PyV system, the inhibitory effects of mouse p48 on SV40 DNA replication are mediated by N- and C-terminal regions of p48. Thus, a chimeric p48 containing human aa 1 to 128, mouse aa 129 to 320, and human aa 321 to 418 is active in both PyV and SV40 DNA replication in vitro.

摘要

DNA聚合酶α-引发酶（pol-prim）是一种由四个亚基组成的复合物，是小鼠多瘤病毒（PyV）和猿猴病毒40（SV40）DNA复制的主要物种特异性因子。尽管p48是pol-prim中最保守的亚基，但它是体外PyV DNA复制所必需的，但会抑制无细胞体系中的SV40 DNA复制。嵌合人-小鼠p48的产生表明，p48的不同区域参与支持PyV DNA复制和抑制SV40 DNA复制。p48的N端和C端部分在无细胞PyV DNA复制中没有物种特异性功能，但中央部分（氨基酸[aa]129至320）在体外控制PyV DNA复制。然而，PyV T抗原无论是否支持PyV DNA复制，都能独立地与小鼠、人及嵌合pol-prim复合物物理结合。与PyV系统不同，小鼠p48对SV40 DNA复制的抑制作用是由p48的N端和C端区域介导的。因此，包含人aa 1至128、小鼠aa 129至320和人aa 321至418的嵌合p48在体外PyV和SV40 DNA复制中均具有活性。