Kramer R M, Holdaway I M, Crighton D B, McNeilly A S, Rees L H, Chard T
J Endocrinol. 1976 May;69(2):205-11. doi: 10.1677/joe.0.0690205.
The cytochemical (redox) bioassay for LH has been compared with established LH assays. Measurements made by redox bioassays were considerably lower than those made by radioimmunoassay in human female plasma samples obtained at mid-cycle. There was no apparent relationship between measurements on incubation media from cultures of sheep pituitary glands made by redox bioassay and the ovarian ascorbic acid depletion (OAAD) assay. After polyacrylamide gel electrophoresis of a crude extract of a human pituitary gland, redox LH measurements were lower than those of the OAAD assay and radioimmunoassay in the cathodal segments of the gel. By contrast, there was reasonable agreement between LH measurements made by radioimmunoassay and redox assay in cathodal fractions from gel electrophroesis of a purified pituitary LH preparation. Follicle-stimulating hormone, and the alpha- and beta-subunits of LH depressed the response of intact LH in the redox assay; this might explain the relatively low levels of LH measured by redox assay in some of the experiments described. Which type of assay best reflects the biological activity of LH in man remains to be determined.
已将促黄体生成素（LH）的细胞化学（氧化还原）生物测定法与既定的LH测定法进行了比较。在人类女性月经周期中期采集的血浆样本中，氧化还原生物测定法的测量值明显低于放射免疫测定法的测量值。氧化还原生物测定法对绵羊垂体培养物的孵育培养基的测量值与卵巢抗坏血酸耗竭（OAAD）测定法之间没有明显关系。对人垂体粗提物进行聚丙烯酰胺凝胶电泳后，凝胶阴极部分的氧化还原LH测量值低于OAAD测定法和放射免疫测定法。相比之下，对纯化的垂体LH制剂进行凝胶电泳后，放射免疫测定法和氧化还原测定法对阴极部分的LH测量值有合理的一致性。促卵泡生成素以及LH的α和β亚基在氧化还原测定中会抑制完整LH的反应；这可能解释了在所述的一些实验中氧化还原测定法测得的LH水平相对较低的原因。哪种测定法最能反映人类LH的生物活性仍有待确定。
