一种用于测定生物体液和组织中促黄体生成素的细胞化学生物测定方法。
A cytochemical bioassay method for the determination of luteinizing hormone in biological fluids and tissues.
作者信息
Buckingham J C, Hodges J R
出版信息
Br J Pharmacol. 1981 May;73(1):111-8. doi: 10.1111/j.1476-5381.1981.tb16779.x.
Abstract
1 The ability of luteinizing hormone (LH) to alter the reducing activity of corpora lutea in rat ovarian sections has been exploited to develop a new cytochemical bioassay for the hormone. 2 Sections of ovaries, removed from mature rats during the second day of dioestrus, were incubated with either standard LH or samples diluted for assay, stained immediately for reducing potential and the intensity of the stain measured by scanning and integrating microdensitometry. 3 An inverse linear relationship existed between the density of the stain and the logarithm of the concentration of standard LH (68/40) and the dose-response lines of serial dilutions of rat or human plasma were parallel with those of the standard. 4 The method was found to be accurate, specific, sensitive, precise and suitable for the determination of LH in the rat and in man.
摘要
- 促黄体生成素（LH）改变大鼠卵巢切片中黄体还原活性的能力已被用于开发一种针对该激素的新细胞化学生物测定法。2. 从处于动情间期第二天的成熟大鼠身上取出卵巢切片，与标准LH或稀释用于测定的样品一起孵育，立即对还原电位进行染色，并通过扫描和积分显微密度测定法测量染色强度。3. 染色密度与标准LH浓度的对数之间存在反向线性关系（68/40），大鼠或人血浆系列稀释液的剂量反应线与标准品的平行。4. 该方法被发现准确、特异、灵敏、精密且适用于测定大鼠和人体中的LH。
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A cytochemical bioassay method for the determination of luteinizing hormone in biological fluids and tissues.一种用于测定生物体液和组织中促黄体生成素的细胞化学生物测定方法。
Br J Pharmacol. 1981 May;73(1):111-8. doi: 10.1111/j.1476-5381.1981.tb16779.x.
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本文引用的文献
1
Plasma levels of luteinizing hormone in women and in rats as measured by bioassay.通过生物测定法测量的女性和大鼠体内促黄体生成素的血浆水平。
J Endocrinol. 1972 Jul;54(1):119-23. doi: 10.1677/joe.0.0540119.
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Applications of the redox bioassay for luteinizing hormone.氧化还原生物测定法在促黄体生成素中的应用。
Clin Endocrinol (Oxf). 1974 Jul;3(3):383-8. doi: 10.1111/j.1365-2265.1974.tb01813.x.
3
Discrepancies in plasma LH activities as measured by radioimmunoassay and an in vitro bioassay.通过放射免疫测定法和体外生物测定法测得的血浆促黄体生成素活性的差异。
Acta Endocrinol (Copenh). 1974 Dec;77(4):672-85. doi: 10.1530/acta.0.0770672.
4
A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: testosterone production by dispersed Leydig cells.一种用于促黄体生成素和绒毛膜促性腺激素的高灵敏度体外生物测定法：分散的睾丸间质细胞产生睾酮。
J Clin Endocrinol Metab. 1974 Sep;39(3):610-3. doi: 10.1210/jcem-39-3-610.
5
The cytochemical section assay for corticotrophin.促肾上腺皮质激素的细胞化学切片检测
Clin Endocrinol (Oxf). 1974 Jul;3(3):319-27. doi: 10.1111/j.1365-2265.1974.tb01805.x.
6
New bioassay for luteinizing hormone.促黄体生成素的新生物测定法。
Nature. 1973 Jul 27;244(5413):232-4. doi: 10.1038/244232a0.
7
A sensitive bioassay for adrenocorticotrophic hormone in human plasma.一种用于检测人血浆中促肾上腺皮质激素的灵敏生物测定法。
Clin Endocrinol (Oxf). 1972 Jul;1(3):219-33. doi: 10.1111/j.1365-2265.1972.tb00393.x.
8
Methodological refinements in the redox bioassay for adrenocorticotrophin.
J Endocrinol. 1973 Jul;58(1):xix.
9
Comparison of the redox bioassay with other assays for luteinizing hormone.氧化还原生物测定法与其他促黄体生成素测定法的比较。
J Endocrinol. 1976 May;69(2):205-11. doi: 10.1677/joe.0.0690205.
10
Biologically active luteinizing hormone (LH) in plasma. 1. Validation of the in vitro bioassay when applied to plasma of women.血浆中的生物活性促黄体生成素（LH）。1. 体外生物测定法应用于女性血浆时的验证。
Acta Endocrinol (Copenh). 1976 Nov;83(3):454-65.
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