Chinchai Teeraporn, Labout Joost, Noppornpanth Suwanna, Theamboonlers Apiradee, Haagmans Bart L, Osterhaus Albert D M E, Poovorawan Yong
Inter-Department of Medical Microbiology, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand.
J Virol Methods. 2003 May;109(2):195-201. doi: 10.1016/s0166-0934(03)00071-5.
Hepatitis C virus (HCV) genotype 6a is found frequently in Southeast Asia. In Thailand, however, genotype 6 variants may exist which posses a genotype 1 like sequence in the 5' non-coding region. In order to genotype correctly these viruses, four different methods; INNO-LiPA assay, two RFLP assays on the core region (using different restriction enzymes) and phylogenetic analysis of the core sequences were compared. Samples from 17 chronic HCV patients from the Netherlands and Thailand and 18 anti-HCV positive blood donors recruited from Thailand were tested. The INNO-LiPA could not distinguish genotype 6 variants. The RFLP methods used could not, or only in combination with 5'NCR genotyping methods, identify type 6 variants. In conclusion, for identification of type 6 variants at least two different regions of the HCV genome have to be analyzed (both 5'NCR and core).
丙型肝炎病毒（HCV）6a基因型在东南亚地区很常见。然而，在泰国，可能存在6型变体，其5'非编码区具有类似1型的序列。为了正确鉴定这些病毒的基因型，比较了四种不同的方法：INNO-LiPA检测法、两种针对核心区域的限制性片段长度多态性（RFLP）检测法（使用不同的限制性内切酶）以及核心序列的系统发育分析。对来自荷兰和泰国的17例慢性HCV患者以及从泰国招募的18例抗HCV阳性献血者的样本进行了检测。INNO-LiPA检测法无法区分6型变体。所使用的RFLP方法无法单独，或仅与5'非编码区基因分型方法结合来鉴定6型变体。总之，为了鉴定6型变体，至少必须分析HCV基因组的两个不同区域（5'非编码区和核心区）。
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