Altindiş Mustafa, Aktepe Orhan Cem, Cetinkaya Zafer, Ciftçi Ihsan Hakki
Afyon Kocatepe Universitesi Tip Fakültesi, Mikrobiyoloji Anabilim Dali, Afyonkarahisar.
Mikrobiyol Bul. 2007 Jan;41(1):121-6.
The detection of genotypes of hepatitis C virus (HCV) which exhibit very high genetic variability, has a great impact for the therapy and follow-up of the chronicity of infections. The aim of this study was to detect the genotypes of HCV strains by using two different methods. Thirty patients (5 hemodialysis patients, 9 chronic hepatitis C patients, 5 blood donors, 1 hospital staff) who were positive for both anti-HCV (Vitros, Ortho-Clinical Diagnostics) and HCV-RNA (Rotorgene, Artus) were included to the study. The serum samples were studied by Inno-LIPA (Inno-LIPA HCV-II, Innogenetics, Belgium) and sequence analysis (9700 Sequence Detection System, and ABI PRISM 310 Genetic Analyzer, Applied Biosystems, USA) methods. For Inno-LIPA, 5'non-coding region (5'NCR) of HCV-RNA was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and genotyped by line probes. For sequence analysis (SA), NS5B and 5'NCR regions were amplified by RT-PCR, and genotypic variations were assessed by Cycle Sequencing system (Applied Biosystems, USA). As a result, one strain was found as 1a, and 28 strains were found as 1b with both Inno-LIPA and SA methods, however, one strain was genotyped as 1b/3a by Inno-LIPA, but as 1b by SA method. Our data have indicated that the results obtained by Inno-LIPA and sequence analysis methods were in concordance for the detection of HCV genotypes, considering that they have similar sensitivities.
丙型肝炎病毒（HCV）基因型具有很高的遗传变异性，其检测对慢性感染的治疗和随访有重大影响。本研究的目的是使用两种不同方法检测HCV毒株的基因型。30名患者（5名血液透析患者、9名慢性丙型肝炎患者、5名献血者、1名医院工作人员）纳入研究，这些患者抗-HCV（Vitros，奥森多临床诊断公司）和HCV-RNA（Rotorgene，雅图公司）均呈阳性。血清样本采用Inno-LIPA（Inno-LIPA HCV-II，比利时Innogenetics公司）和序列分析（9700序列检测系统以及美国应用生物系统公司的ABI PRISM 310基因分析仪）方法进行研究。对于Inno-LIPA，通过逆转录聚合酶链反应（RT-PCR）扩增HCV-RNA的5'非编码区（5'NCR），并通过线性探针进行基因分型。对于序列分析（SA），通过RT-PCR扩增NS5B和5'NCR区域，并通过循环测序系统（美国应用生物系统公司）评估基因变异。结果，通过Inno-LIPA和SA方法均发现1株为1a型，28株为1b型，然而，1株通过Inno-LIPA基因分型为1b/3a型，但通过SA方法为1b型。我们的数据表明，考虑到Inno-LIPA和序列分析方法具有相似的灵敏度，它们在检测HCV基因型方面的结果是一致的。
