Moore H D, Hall G A, Hibbitt K G
J Reprod Fertil. 1976 May;47(1):39-45. doi: 10.1530/jrf.0.0470039.
Spermatozoa from intact boars and from boars without seminal vesicles were resuspected in diluent and cooled at different rates to 0 degrees C. Glutamic oxaloacetic transaminase and lactate dehydrogenase activities were greater in the diluents which had contained spermatozoa from intact boars than in those which contained spermatozoa from animals without seminal vesicles. The incubation of seminal plasma from an intact boar with spermatozoa from a vesiculectomized animal before cooling also increased the enzyme activity in the diluent. The factors responsible for this effect were associated with the basic protein fractions of boar seminal plasma, in particular the proteins with haemagglutinating activity which may have been adsorbed onto the spermatozoa. Spermatozoa were exposed to colloidal Fe(OH)2+ to determine by electron microscopy the charge on the surface of the plasma membrane of washed epididymal spermatozoa and ejaculated spermatozoa from intact and vesiculectomized boars. Epididymal spermatozoa bound the positively charged particles more readily than the ejaculated spermatozoa from the intact boars, due to the absence of membrane-bound protein.
将完整公猪和无精囊公猪的精子在稀释液中复苏，并以不同速率冷却至0摄氏度。含有完整公猪精子的稀释液中谷草转氨酶和乳酸脱氢酶的活性，高于含有无精囊公猪精子的稀释液。在冷却前，将完整公猪的精浆与去精囊动物的精子一起孵育，也会增加稀释液中的酶活性。造成这种效应的因素与公猪精浆的碱性蛋白组分有关，特别是具有血凝活性的蛋白质，这些蛋白质可能已吸附在精子上。将精子暴露于胶体Fe(OH)₂⁺，通过电子显微镜确定来自完整和去精囊公猪的附睾洗涤精子和射精精子质膜表面的电荷。附睾精子比来自完整公猪的射精精子更容易结合带正电荷的颗粒，这是由于缺乏膜结合蛋白。
