[Identification of myeloid dendritic cells in peritoneal fluid by flow cytometry].

The myeloid lineage-derived DCs are the most potent antigen-presenting cells (APC), which are highly specialized in capturing antigens and triggering adaptive immune responses. The purpose of our study was to identify of the myeloid DCs in peritoneal fluid of women with nonmalignant ovarian tumors. With use of flow cytometry we detected myeloid dendritic cells in peritoneal fluid of the studied patients. Myeloid DCs were identified as a discrete population of mononuclear cells expressing high levels of HLA-DR and CD33, but without expression of CD14 and CD16. The number of DCs was shown as a percentage of mononuclear cells. Dendritic cells comprised 3.02% (0.35%-8.41%) in patients with ovarian tumors and 1.72% (0.51%-6.2%) of peritoneal fluid mononuclear cells in women with unexplained infertility. The percentage of DCs in peritoneal fluid was significantly higher than in peripheral blood.