Wertel Iwona, Kotarski Jan, Roliński Jacek, Bojarska-Junak Agnieszka, Gogacz Marek
Department of Gynecology, University School of Medicine, ul. Staszica 16, 20-081 Lublin, Poland.
Am J Reprod Immunol. 2003 Sep;50(3):238-42. doi: 10.1034/j.1600-0897.2003.00081.x.
Identification of myeloid and lymphoid dendritic cells (DCs) in peritoneal fluid (PF) and peripheral blood (PB) of patients with ovarian pathology.
PF and PB were collected from 60 patients who underwent laparoscopy because of non-malignant ovarian tumors. Mononuclear cells were separated by gradient centrifugation. The cell surface antigens were determined by flow cytometry using monoclonal antibodies.
Both myeloid and lymphoid DCs were detected in PF and PB of women with ovarian tumors. The percentage of myeloid DCs was significantly higher in PF than in PB. The concentration of PF myeloid DCs was the highest (P < 0.05) in patients with dermoid cysts (0.67 x 10(6)/mL PF) in comparison with the other studied groups, excluding patients with normal pelvis.
Domination of myeloid and not lymphoid cells in PF may support the hypothesis that local PF immune disturbances may play a role in some non-malignant ovarian pathology.
鉴定卵巢病变患者腹腔液（PF）和外周血（PB）中的髓样和淋巴样树突状细胞（DCs）。
收集60例因非恶性卵巢肿瘤接受腹腔镜检查患者的PF和PB。通过梯度离心分离单核细胞。使用单克隆抗体通过流式细胞术测定细胞表面抗原。
在患有卵巢肿瘤的女性的PF和PB中均检测到髓样和淋巴样DCs。PF中髓样DCs的百分比显著高于PB。与其他研究组相比，皮样囊肿患者（0.67×10⁶/mL PF）的PF髓样DCs浓度最高（P<0.05），不包括骨盆正常的患者。
PF中髓样而非淋巴样细胞占主导可能支持以下假设，即局部PF免疫紊乱可能在某些非恶性卵巢病变中起作用。
