Brewer C F, Keiser H
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3421-3. doi: 10.1073/pnas.72.9.3421.
The Fourier transform 13C nuclear magnetic resonance spectra of bovine nasal cartilage proteoglycan subunit and complex and whole bovine nasal cartilage were obtained and compared with that of chondroitin 4-sulfate. The spectrum of chondroitin 4-sulfate in solution revealed multiple resolvable resonances with linewidths that are consistent with considerable segmental motion in the polysaccharide chain. The spectra of proteoglycan subunit and complex in solution and that of whole cartilage were very similar to that of free chondroitin 4-sulfate chains. This indicates that the linkage of multiple chondroitin sulfate chains to proteoglycan core protein and the association of proteoglycan with collagen and other constituents of cartilage matrix does not significantly alter the structure and motions of these chains.
获得了牛鼻软骨蛋白聚糖亚基、复合物以及完整牛鼻软骨的傅里叶变换13C核磁共振谱，并与4-硫酸软骨素的谱进行了比较。溶液中4-硫酸软骨素的谱显示出多个可分辨的共振峰，其线宽与多糖链中相当程度的片段运动一致。溶液中蛋白聚糖亚基和复合物以及完整软骨的谱与游离4-硫酸软骨素链的谱非常相似。这表明多条硫酸软骨素链与蛋白聚糖核心蛋白的连接以及蛋白聚糖与胶原蛋白和软骨基质其他成分的缔合并没有显著改变这些链的结构和运动。
