人浸润性乳腺癌的组织学分级、HsMCM2表达与细胞周期蛋白A之间的关联。
Association between histology grade, expression of HsMCM2, and cyclin A in human invasive breast carcinomas.
作者信息
Bukholm I R K, Bukholm G, Holm R, Nesland J M
机构信息
Department of Surgery, Akershus University Hospital, 1474 Nordbyhagen, Norway.
出版信息
J Clin Pathol. 2003 May;56(5):368-73. doi: 10.1136/jcp.56.5.368.
AIM
Increased proliferation of tumour cells has prognostic value in human invasive breast carcinomas (IBCs), and high histology grade and cyclin A expression, which may reflect high proliferation rate, are associated with poor prognosis. Expression of HsMCM2 is related to cell proliferation. This study evaluates the correlation between the expression of cyclins A, D1, D3, and E, Ki-67, proliferating cell nuclear antigen (PCNA), histology grade, and HsMCM2 expression, in addition to the independent prognostic value of HsMCM2 expression in human IBCs.
METHODS
Immunohistochemistry to evaluate HsMCM2, Ki-67, and PCNA expression in tumours from 147 patients with IBC.
RESULTS
Nuclear staining for HsMCM2 was seen in 10-30% of the tumour cells in 30 samples, in 30-70% in 40 samples, in > 70% in 44 samples, and in < 10% in 33 samples. One way ANOVA showed a significant association between expression of HsMCM2 and cyclin A, D3, E, histology grade, and Ki-67. A borderline correlation was seen between HsMCM2 and PCNA. In multivariate analysis, the only association was with cyclin A, in addition to a borderline association with histology grade. In a Cox regression hazards model, expression of HsMCM2 was associated with poor patient survival, although it lost its independent prognostic value when cyclin A expression was included. Ki-67 and PCNA expression were not associated with patient survival.
CONCLUSION
Cyclin A expression is independently associated with HsMCM2 expression, histology grade, and Ki-67. HsMCM2 expression is associated with poor patient survival, although it loses prognostic value when adjusted for cyclin A.
目的
肿瘤细胞增殖增加在人类浸润性乳腺癌（IBC）中具有预后价值，而高组织学分级和细胞周期蛋白A表达可能反映高增殖率，与预后不良相关。HsMCM2的表达与细胞增殖有关。本研究评估细胞周期蛋白A、D1、D3和E、Ki-67、增殖细胞核抗原（PCNA）的表达、组织学分级与HsMCM2表达之间的相关性，以及HsMCM2表达在人类IBC中的独立预后价值。
方法
采用免疫组织化学法评估147例IBC患者肿瘤中HsMCM2、Ki-67和PCNA的表达。
结果
30份样本中10%-30%的肿瘤细胞可见HsMCM2核染色，40份样本中为30%-70%，44份样本中>70%，33份样本中<10%。单因素方差分析显示HsMCM2表达与细胞周期蛋白A、D3、E、组织学分级和Ki-67之间存在显著相关性。HsMCM2与PCNA之间存在边缘相关性。在多因素分析中，唯一的关联是与细胞周期蛋白A，此外与组织学分级存在边缘关联。在Cox回归风险模型中，HsMCM2的表达与患者生存率差相关，尽管当纳入细胞周期蛋白A表达时它失去了独立的预后价值。Ki-67和PCNA表达与患者生存率无关。
结论
细胞周期蛋白A表达与HsMCM2表达、组织学分级和Ki-67独立相关。HsMCM2表达与患者生存率差相关，尽管在调整细胞周期蛋白A后它失去了预后价值。
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