微小染色体维持（MCM）蛋白与RNA聚合酶II全酶相关联。
MCM proteins are associated with RNA polymerase II holoenzyme.
作者信息
Yankulov K, Todorov I, Romanowski P, Licatalosi D, Cilli K, McCracken S, Laskey R, Bentley D L
机构信息
Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
出版信息
Mol Cell Biol. 1999 Sep;19(9):6154-63. doi: 10.1128/MCB.19.9.6154.
MCMs are a family of proteins related to ATP-dependent helicases that bind to origin recognition complexes and are required for initiation of DNA replication. We report that antibodies against MCM2(BM28) specifically inhibited transcription by RNA polymerase II (Pol II) in microinjected Xenopus oocytes. Consistent with this observation, MCM2 and other MCMs copurified with Pol II and general transcription factors (GTFs) in high-molecular-weight holoenzyme complexes isolated from Xenopus oocytes and HeLa cells. Pol II and GTFs also copurified with MCMs isolated by anti-MCM3 immunoaffinity chromatography. MCMs were specifically displaced from the holoenzyme complex by antibody against the C-terminal domain (CTD) of Pol II. In addition, MCMs bound to a CTD affinity column, suggesting that their association with holoenzyme depends in part on this domain of Pol II. These results suggest a new function for MCM proteins as components of the Pol II transcriptional apparatus.
微小染色体维持蛋白（MCMs）是一类与ATP依赖性解旋酶相关的蛋白质，它们与起始识别复合物结合，是DNA复制起始所必需的。我们报道，针对MCM2（BM28）的抗体在显微注射的非洲爪蟾卵母细胞中特异性抑制RNA聚合酶II（Pol II）的转录。与这一观察结果一致，在从非洲爪蟾卵母细胞和HeLa细胞中分离出的高分子量全酶复合物中，MCM2和其他MCMs与Pol II和通用转录因子（GTFs）共同纯化。Pol II和GTFs也与通过抗MCM3免疫亲和层析分离的MCMs共同纯化。通过针对Pol II C端结构域（CTD）的抗体，MCMs从全酶复合物中被特异性置换。此外，MCMs与CTD亲和柱结合，表明它们与全酶的结合部分取决于Pol II的这一结构域。这些结果表明MCM蛋白作为Pol II转录装置的组成部分具有新功能。
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