Tabata Makiko, Shimizu Masaki, Araake Minako, Ogawa Hiroshi
Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., 760 Morooka-cho, Kohoku-ku, Yokohama, Kanagawa 222-8567, Japan.
Jpn J Antibiot. 2003 Feb;56(1):36-43.
The susceptibility to arbekacin (ABK) of methicillin-resistant Staphylococcus aureus (MRSA) was investigated to find out how it related to aac(6')/aph(2") gene. In 49 isolates of MRSA for which MIC of ABK ranged from 0.125 to 64 micrograms/ml, the MICs of ABK for 38 strains carrying aac(6')/aph(2") gene were widely distributed from 0.25 to 64, whereas those for 11 strains without that gene were all < or = 0.5 microgram/ml. Residual rate of ABK activity was higher than that of gentamicin after the reaction with each crude enzyme preparation extracted from 3 isolates of MRSA, carrying aac(6')/aph(2") and aad(4',4") genes. Furthermore, 97 strains of MRSA isolated at Kanagawa prefecture in Japan in 1999 were all sensitive to ABK, although 28 strains of them carried aac(6')/aph(2") gene. These results showed that ABK resistance was not necessarily related to carrying aac(6')/aph(2") gene in clinical isolates of MRSA.
研究了耐甲氧西林金黄色葡萄球菌（MRSA）对阿贝卡星（ABK）的敏感性，以了解其与aac(6')/aph(2")基因的关系。在49株ABK最低抑菌浓度（MIC）范围为0.125至64微克/毫升的MRSA分离株中，携带aac(6')/aph(2")基因的38株菌株的ABK MIC广泛分布于0.25至64，而11株无该基因菌株的MIC均≤0.5微克/毫升。与从携带aac(6')/aph(2")和aad(4',4")基因的3株MRSA分离株中提取的每种粗酶制剂反应后，ABK活性残留率高于庆大霉素。此外，1999年在日本神奈川县分离的97株MRSA均对ABK敏感，尽管其中28株携带aac(6')/aph(2")基因。这些结果表明，在MRSA临床分离株中，ABK耐药性不一定与携带aac(6')/aph(2")基因有关。
