Kitayama Shigeru, Narumi Issay, Funayama Tomoo, Watanabe Hiroshi
Cell Physiology Laboratory, The Institute of Physical & Chemical Research, Wako, Saitama 350-0198, Japan.
Biosci Biotechnol Biochem. 2003 Mar;67(3):613-6. doi: 10.1271/bbb.67.613.
To characterize its enzymic property we cloned and sequenced the gene of Deinococcus radiodurans encoding UV-endonuclease beta, an alternative enzyme to UvrABC repairing damaged DNA. Amino acid substitutions were found in UV-sensitive mutants. The putative amino acid sequence had some similarity with those of eukaryotic UV-endonucleases and with a sequence found in a protein data base of Bacillus subtilis.
为了表征其酶学特性，我们克隆并测序了耐辐射球菌编码紫外线内切酶β的基因，该酶是UvrABC修复受损DNA的替代酶。在紫外线敏感突变体中发现了氨基酸取代。推测的氨基酸序列与真核生物紫外线内切酶的序列以及枯草芽孢杆菌蛋白质数据库中发现的一个序列有一些相似性。
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