Zhimulev Igor F, Belyaeva Elena S, Makunin Igor V, Pirrotta Vincenzo, Semeshin Valery F, Alekseyenko Artyom A, Belyakin Stepan N, Volkova Elena I, Koryakov Dmitry E, Andreyeva Evgeniya N, Demakova Olga V, Kotlikova Irina V, Kolesnikova Tatyana D, Boldyreva Lidiya V, Nanayev Roman A
Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Lavrentyev Ave., 10, 630090, Novosibirsk, Russia.
Genetica. 2003 Mar;117(2-3):259-70. doi: 10.1023/a:1022912716376.
The morphological characteristics of intercalary heterochromatin (IH) are compared with those of other types of silenced chromatin in the Drosophila melanogaster genome: pericentric heterochromatin (PH) and regions subject to position effect variegation (PEV). We conclude that IH regions in polytene chromosomes are binding sites of silencing complexes such as PcG complexes and of SuUR protein. Binding of these proteins results in the appearance of condensed chromatin and late replication of DNA, which in turn may result in DNA underreplication. IH and PH as well as regions subject to PEV have in common the condensed chromatin appearance, the localization of specific proteins, late replication, underreplication in polytene chromosomes, and ectopic pairing.
将果蝇基因组中居间异染色质（IH）的形态特征与其他类型的沉默染色质：着丝粒周围异染色质（PH）和受位置效应斑驳（PEV）影响的区域进行比较。我们得出结论，多线染色体中的IH区域是沉默复合物（如PcG复合物）和SuUR蛋白的结合位点。这些蛋白质的结合导致染色质浓缩和DNA复制延迟，进而可能导致DNA复制不足。IH和PH以及受PEV影响的区域共同具有染色质浓缩的外观、特定蛋白质的定位、复制延迟、多线染色体中的复制不足以及异位配对。
