果蝇黑腹果蝇异染色质h39区域中Bari-1簇的组织及可能起源

Organization and possible origin of the Bari-1 cluster in the heterochromatic h39 region of Drosophila melanogaster.

作者信息

Marsano René Massimiliano, Milano Rosabella, Minervini Crescenzio, Moschetti Roberta, Caggese Corrado, Barsanti Paolo, Caizzi Ruggiero

机构信息

Dipartimento di Anatomia Patologica e di Genetica, Sezione di Genetica, Università di Bari. Via G. Amendola 165/A, 70126 Bari, Italy.

出版信息

Genetica. 2003 Mar;117(2-3):281-9. doi: 10.1023/a:1022916817285.

DOI:10.1023/a:1022916817285

PMID:12723707

Abstract

The molecular organization of the heterochromatic h39 region of the Drosophila melanogaster second chromosome has been investigated by studying two BAC clones identified both by Southern blotting and by FISH experiments as containing tandem arrays of Bari1, a transposable element present only in this region. Such BAC clones appear to contain different portions of the h39 region since they differ in the DNA sequences flanking the Bari1 repeats on both sides. Thus, the 80 Bari1 copies estimated to be present in the h39 region are split into at least two separated subregions. On the basis of the analysis of the flanking sequences a possible mechanism depending on an aberrant activity of the Bari1 transposase is proposed for the genesis of the heterochromatic tandem arrays of the element.

摘要

通过研究两个经Southern印迹法和荧光原位杂交实验鉴定的细菌人工染色体（BAC）克隆，对黑腹果蝇第二条染色体异染色质h39区域的分子组织进行了研究。这两个克隆均含有Bari1的串联阵列，Bari1是一种仅存在于该区域的转座元件。由于这两个BAC克隆在Bari1重复序列两侧的DNA序列不同，它们似乎包含h39区域的不同部分。因此，估计存在于h39区域的80个Bari1拷贝被分成至少两个分开的亚区域。基于侧翼序列分析，提出了一种可能依赖于Bari1转座酶异常活性的机制，用于解释该元件异染色质串联阵列的起源。