Bastien Nathalie, Normand Susan, Taylor Tracy, Ward Diane, Peret Teresa C T, Boivin Guy, Anderson Larry J, Li Yan
National Microbiology Laboratory, Canadian Science Center for Human and Animal Health, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2.
Virus Res. 2003 May;93(1):51-62. doi: 10.1016/s0168-1702(03)00065-0.
The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M), and fusion protein (F) genes of 15 Canadian human metapneumovirus (hMPV) isolates were determined. Phylogenetic analysis revealed two distinct genetic clusters, or groups for each gene with additional sequence variability within the individual groups. Comparison of the deduced amino acid sequences for the N, M and F genes of the different isolates revealed that all three genes were well conserved with 94.1-97.6% identity between the two distinct clusters The P gene showed more diversity with 81.6-85.7% amino acid identity for isolates between the two clusters, and 94.6-100% for isolates within the same cluster.
测定了15株加拿大人间偏肺病毒（hMPV）分离株的核蛋白（N）、磷蛋白（P）、基质蛋白（M）和融合蛋白（F）基因的完整核苷酸序列。系统发育分析揭示了两个不同的遗传簇，或每个基因的两个组，且各个组内存在额外的序列变异性。对不同分离株的N、M和F基因推导的氨基酸序列进行比较，结果显示所有这三个基因都高度保守，两个不同簇之间的同一性为94.1%-97.6%。P基因表现出更多样性，两个簇之间的分离株氨基酸同一性为81.6%-85.7%，同一簇内的分离株氨基酸同一性为94.6%-100%。
