Langeggen Harald, Namork Ellen, Johnson Egil, Hetland Geir
Research Forum and Department of Pediatrics, Ullevål University Hospital, Kirkevej Kirkeveien 166, 0404 Oslo, Norway.
FEMS Immunol Med Microbiol. 2003 May 15;36(1-2):55-61. doi: 10.1016/S0928-8244(03)00033-6.
Uptake of zymosan A particles by human umbilical vein endothelial cells (HUVEC) and its effect on cellular cytokine and oxygen radical production was examined. HUVEC took up more serum-opsonized than -unopsonized zymosan as demonstrated by flow cytometry with fluorescence-labeled particles. The former uptake was inhibited in the presence of anti-C3c antibodies and thus complement-mediated. It probably occurred via CR1 (CD35), although participation of other receptors cannot be ruled out. Scanning electron microscopy indicated that HUVEC with fully internalized zymosan particles were damaged. Prolonged incubation of both serum-opsonized and -unopsonized zymosan particles with HUVEC induced increased secretion of the proinflammatory cytokines IL-6 and IL-8 to the cell culture supernatants, but had no effect on production of oxygen radicals. The results confirm previous reports that EC can internalize yeast and other pathogens and points to complement as a mechanism of uptake, but illustrates that the cells may be damaged in the process. Moreover, EC may participate in the anti-infection defense effort by secreting proinflammatory and chemotactic cytokines in response to the contact with pathogens.
研究了人脐静脉内皮细胞（HUVEC）对酵母聚糖A颗粒的摄取及其对细胞因子产生和氧自由基生成的影响。通过使用荧光标记颗粒的流式细胞术表明，HUVEC摄取血清调理素化的酵母聚糖比未调理素化的更多。在抗C3c抗体存在的情况下，前者的摄取受到抑制，因此是补体介导的。尽管不能排除其他受体的参与，但它可能通过CR1（CD35）发生。扫描电子显微镜显示，酵母聚糖颗粒完全内化的HUVEC受到损伤。血清调理素化和未调理素化的酵母聚糖颗粒与HUVEC长时间孵育均导致促炎细胞因子IL-6和IL-8分泌增加至细胞培养上清液中，但对氧自由基的生成没有影响。结果证实了先前的报道，即内皮细胞可以内化酵母和其他病原体，并指出补体是一种摄取机制，但表明细胞在此过程中可能会受损。此外，内皮细胞可能通过在与病原体接触时分泌促炎和趋化细胞因子来参与抗感染防御。
