Bernardshaw Soosaipillai, Hetland Geir, Ellertsen Linda Kathrine, Tryggestad Anne Merete Aaland, Johnson Egil
Department of Gastroenterological Surgery, Ulleval University Hospital, Kirkeveien 166, 0407 Oslo, Norway.
Inflammation. 2005 Dec;29(4-6):147-53. doi: 10.1007/s10753-006-9010-2.
An extract of the edible mushroom Agaricus blazei Murill (AbM) has known antitumor and anti-infection properties, probably mainly by stimulating mononuclear phagocytes of the native immune system. The aim of this work was to study the effect of AbM on the production by human monocytes and human umbilical vein endothelial cells (EC) of pro-inflammatory cytokines (IL-1beta, IL-6, IL-8, TNFalpha), the anti-inflammatory/T regulatory cytokine IL-10 and the pro-Th1 cytokine IL-12. AbM, in concentrations from 1-15%, induced a considerable and dose-dependent increase in production of IL-8, IL-6, TNFalpha and IL-1beta in monocyte cultures. The biosynthesis reached a plateau at a concentration of 10% of AbM, and was most pronounced for the three former cytokines. AbM did also dose-dependently stimulate EC production of IL-8,I L-6 and TNFalpha, but at lower levels compared with the monocytes. AbM did neither induce synthesis of cytokines IL-10 nor IL-12 in monocytes or EC. Our results demonstrate the differential effect of AbM stimulation on the magnitude of pro-inflammatory cytokines produced by monocytes and EC.
食用蘑菇姬松茸（AbM）的提取物具有已知的抗肿瘤和抗感染特性，可能主要是通过刺激天然免疫系统的单核吞噬细胞。这项工作的目的是研究AbM对人单核细胞和人脐静脉内皮细胞（EC）产生促炎细胞因子（IL-1β、IL-6、IL-8、TNFα）、抗炎/T调节细胞因子IL-10和促Th1细胞因子IL-12的影响。浓度为1%-15%的AbM可诱导单核细胞培养物中IL-8、IL-6、TNFα和IL-1β的产生显著且呈剂量依赖性增加。生物合成在AbM浓度为10%时达到平台期，对前三种细胞因子最为明显。AbM也呈剂量依赖性刺激EC产生IL-8、IL-6和TNFα，但与单核细胞相比水平较低。AbM既不诱导单核细胞或EC中细胞因子IL-10也不诱导IL-12的合成。我们的结果证明了AbM刺激对单核细胞和EC产生的促炎细胞因子数量的不同影响。
