Ding Jin, Nagai Kazuo, Woo Je-Tae
Department of Bioscience and Biochemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumotomachi, Kasugai-shi, Aichi-ken 487-8501, Japan.
Biosci Biotechnol Biochem. 2003 Feb;67(2):314-21. doi: 10.1271/bbb.67.314.
ST2 cells, a cloned stromal-cell line from mouse bone marrow have the phenotypes of osteoblasts and hematopoietic supporting cells, but little is known about the adipogenesis in this cell line. We found that treatment of ST2 cells with a cocktail, a combination of insulin, dexamethasone (DEX) and 3-isobutyl-1-methylxanthine (IBMX), induced adipocyte differentiation. The cells were adipocytic, as seen by the accumulation of triglycerides and with lipid droplets stained with Oil Red O. Expressions of the adipogenic transcriptional factors peroxisome-proliferator-activated receptor gamma and CCAAT-enhancer binding protein alpha were stimulated, triggering the expression of the late adipocyte-specific marker alpha-glycerophosphate-3-dehydrogenase. Unlike another bone marrow stromal cell line PA6, ST2 cells responded to the adipogenic effects of insulin, as do the extramedullary preadipocytes 3T3-L1. Like PA6 and 3T3-L1 cells, adipogenesis in ST2 cells was inhibited by 1,25-dihydroxyvitamin D3, retinoic acid, tumour necrosis factor alpha, and transforming growth factor beta. Therefore, ST2 cells can differentiate into osteoblasts, adipocytes, and hematopoietic supporting cells, in which the process of adipogenesis differs from that of bone marrow preadipocytes PA6, and resembles that of the extramedullary preadipocytes 3T3-L1.
ST2细胞是一种从小鼠骨髓中克隆得到的基质细胞系，具有成骨细胞和造血支持细胞的表型，但关于该细胞系中的脂肪生成知之甚少。我们发现，用胰岛素、地塞米松（DEX）和3-异丁基-1-甲基黄嘌呤（IBMX）的混合物处理ST2细胞可诱导脂肪细胞分化。细胞呈脂肪细胞样，可见甘油三酯积累且脂滴被油红O染色。脂肪生成转录因子过氧化物酶体增殖物激活受体γ和CCAAT增强子结合蛋白α的表达受到刺激，从而触发晚期脂肪细胞特异性标志物α-甘油磷酸脱氢酶的表达。与另一种骨髓基质细胞系PA6不同，ST2细胞对胰岛素的脂肪生成作用有反应，骨髓外前脂肪细胞3T3-L1也是如此。与PA6和3T3-L1细胞一样，ST2细胞中的脂肪生成受到1,25-二羟基维生素D3、视黄酸、肿瘤坏死因子α和转化生长因子β的抑制。因此，ST2细胞可分化为成骨细胞、脂肪细胞和造血支持细胞，其中脂肪生成过程不同于骨髓前脂肪细胞PA6，而类似于骨髓外前脂肪细胞3T3-L1。
