Zhu Ying, Yang Yanwei, Qi Qiping
Institute for Environment Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Wei Sheng Yan Jiu. 2003 Jan;32(1):51-2, 87.
A high performance liquid chromatography(HPLC) was described for the determination of chlorhexidine acetate in disinfectant on a C18 column using acetonitrile-0.02 mol/L potassium dihydrogen phosphate solution(pH2.5) (40:60) as mobile phase. The regression equation was linear when the chlorhexidine acetate concentration was lower than 1.00 g/L. The detection limit was 8 mg/L. The precision was less than 4.0% and the recovery varied from 99.8% to 115.2%. The method was more sensitive, rapid and simple than other HPLC methods, and the characteristic of low disturbance makes it especially important for the assay of chlorhexidine acetate in compound disinfectant comparing with titrimetry and UV spectrophotometry.
描述了一种高效液相色谱法（HPLC），用于测定消毒剂中的醋酸氯己定。该方法采用C18柱，以乙腈-0.02 mol/L磷酸二氢钾溶液（pH2.5）（40:60）作为流动相。当醋酸氯己定浓度低于1.00 g/L时，回归方程呈线性。检测限为8 mg/L。精密度小于4.0%，回收率在99.8%至115.2%之间。该方法比其他HPLC方法更灵敏、快速和简便，与滴定法和紫外分光光度法相比，其低干扰特性对于复方消毒剂中醋酸氯己定的测定尤为重要。
