Goldfinger D, Solis R T, Meryman H T
Blood Bank Department, Clinical Center, National Institutes of Health, Bethesda, Maryland, Walter Reed Army Institute for Research, Washington, D. C. 20014, USA.
Transfusion. 1974 Mar-Apr;14(2):151-4. doi: 10.1111/j.1537-2995.1974.tb04508.x.
The microaggregates which accumulate in stored blood have been implicated in the development of posttraumatic pulmonary insufficiency. These particles are known to be composed of degenerated leukocytes and platelets. Because frozen and saline washed red blood cells contain small numbers of leukocytes and platelets, they were studied as a possible source of microaggregate-free red blood cells. Using a Model T Coulter Counter to quantitate all particles 13-80 microns in size, it was shown that freezing and deglycerolization, or simple saline washing (manual or automated), could reduce the number of microaggregates in stored blood by 80 to 90 per cent. These findings add to a growing list of potential advantages in the routine use of frozen red cells for patients requiring transfusion.
储存血液中积聚的微聚集体与创伤后肺功能不全的发生有关。已知这些颗粒由变性的白细胞和血小板组成。由于冷冻和盐水洗涤的红细胞含有少量白细胞和血小板，因此对它们作为无微聚集体红细胞的可能来源进行了研究。使用型号T库尔特计数器对所有大小在13至80微米的颗粒进行定量，结果表明，冷冻和去除甘油，或简单的盐水洗涤（手动或自动），可使储存血液中的微聚集体数量减少80%至90%。这些发现进一步增加了在需要输血的患者中常规使用冷冻红细胞的潜在优势。
