Borukhov Sergei, Nudler Evgeny
Department of Microbiology and Immunology, SUNY Health Sciences Center, 450 Clarkson Avenue, Room BSB 3-27, Brooklyn, NY 11203, USA.
Curr Opin Microbiol. 2003 Apr;6(2):93-100. doi: 10.1016/s1369-5274(03)00036-5.
The past three years have marked the breakthrough in our understanding of the structural and functional organization of RNA polymerase. The latest major advance was the high-resolution structures of bacterial RNA polymerase holoenzyme and the holoenzyme in complex with promoter DNA. Together with an array of genetic, biochemical and biophysical data accumulated to date, the structures provide a comprehensive view of dynamic interactions between the major components of transcription machinery during the early stages of the transcription cycle. They include the binding of sigma factor to the core enzyme, and the recognition of promoter sequences and DNA melting by holoenzyme, transcription initiation and promoter clearance.
过去三年标志着我们对RNA聚合酶的结构和功能组织的理解取得了突破。最新的重大进展是细菌RNA聚合酶全酶以及与启动子DNA结合的全酶的高分辨率结构。这些结构与迄今为止积累的一系列遗传、生化和生物物理数据一起，提供了转录周期早期转录机制主要成分之间动态相互作用的全面视图。它们包括σ因子与核心酶的结合、全酶对启动子序列的识别和DNA解链、转录起始和启动子清除。
