Rhodius Virgil A, LaRossa Robert A
Department of Microbiology and Immunology, University of California, Genentech Hall, 600 16th Street, San Francisco, CA 94143-2200, USA.
Curr Opin Microbiol. 2003 Apr;6(2):114-9. doi: 10.1016/s1369-5274(03)00034-1.
Microarrays provide a powerful new tool for understanding the regulation of gene expression in bacteria. Many recent publications have used microarrays for identifying regulon members and stimulons that describe the complex organismal responses to environmental perturbations. The use of bioinformatics to identify DNA binding sites of transcription factors greatly facilitates the interpretation of these experiments. Understanding the transcriptome of an organism includes identifying all transcripts and mapping their 5' and 3' ends. High-density oligonucleotide arrays have enabled the identification of many new transcripts, including small RNAs and antisense RNAs.
微阵列提供了一个强大的新工具，用于理解细菌中基因表达的调控。最近许多出版物都使用微阵列来识别调节子成员和刺激子，这些调节子和刺激子描述了生物体对环境扰动的复杂反应。利用生物信息学来识别转录因子的DNA结合位点极大地促进了对这些实验的解释。了解生物体的转录组包括识别所有转录本并绘制它们的5'和3'末端图谱。高密度寡核苷酸阵列已经能够识别许多新的转录本，包括小RNA和反义RNA。
