通过16S rDNA限制性分析确定的属于鞘丝藻属和螺旋藻属的颤藻科菌株的基因型多样性。

Genotypic diversity of oscillatoriacean strains belonging to the genera Geitlerinema and Spirulina determined by 16S rDNA restriction analysis.

作者信息

Margheri Maria C, Piccardi Raffaella, Ventura Stefano, Viti Carlo, Giovannetti Luciana

机构信息

Istituto per lo Studio degli Ecosistemi del CNR, P. le delle Cascine, 28, I-50144 Firenze, Italy.

出版信息

Curr Microbiol. 2003 May;46(5):359-64. doi: 10.1007/s00284-002-3869-4.

DOI:10.1007/s00284-002-3869-4

PMID:12732964

Abstract

Genotypic diversity of several cyanobacterial strains mostly isolated from marine or brackish waters, belonging to the genera Geitlerinema and Spirulina, was investigated by amplified 16S ribosomal DNA restriction analysis and compared with morphological features and response to salinity. Cluster analysis was performed on amplified 16S rDNA restriction profiles of these strains along with profiles obtained from sequence data of five Spirulina-like strains, including three representatives of the new genus Halospirulina. Our strains with tightly coiled trichomes from hypersaline waters could be assigned to the Halospirulina genus. Among the uncoiled strains, the two strains of hypersaline origin clustered together and were found to be distant from their counterparts of marine and freshwater habitat. Moreover, another cluster, formed by alkali-tolerant strains with tightly coiled trichomes, was well delineated.

摘要

对主要从海洋或咸水水域分离出的、属于鞘丝藻属（Geitlerinema）和螺旋藻属（Spirulina）的几种蓝藻菌株的基因型多样性进行了扩增16S核糖体DNA限制性分析，并与形态特征和对盐度的反应进行了比较。对这些菌株的扩增16S rDNA限制性图谱以及从五个螺旋藻样菌株（包括新属嗜盐螺旋藻属（Halospirulina）的三个代表菌株）的序列数据获得的图谱进行了聚类分析。我们从高盐水域分离出的具有紧密盘绕藻丝的菌株可归为嗜盐螺旋藻属。在未盘绕的菌株中，两个来自高盐环境的菌株聚集在一起，且发现它们与海洋和淡水生境的对应菌株相距较远。此外，由具有紧密盘绕藻丝的耐碱菌株形成的另一个聚类也清晰可辨。