Youshko M I, Bukhanov A L, Svedas V K
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992 Russia.
Biochemistry (Mosc). 2003 Mar;68(3):334-8. doi: 10.1023/a:1023014519139.
The influence of the external nucleophile (6-aminopenicillanic acid) on the kinetics of the penicillin acylase-catalyzed acyl transfer reactions was studied using a highly sensitive spectrophotometric assay. An adequate kinetic scheme is suggested based on kinetic analysis of the experimental dependencies of the k(cat) and K(m) values on the nucleophile concentration. The proposed kinetic scheme has been verified by a quantitative description of the above-mentioned experimental dependencies using the set of kinetic parameters obtained from independent experiments. Such an approach can be used for modeling of different penicillin acylase-catalyzed acyl transfer reactions.
使用高灵敏度分光光度法研究了外部亲核试剂（6-氨基青霉烷酸）对青霉素酰化酶催化的酰基转移反应动力学的影响。基于对k(cat)和K(m)值对亲核试剂浓度的实验依赖性进行动力学分析，提出了一个合适的动力学方案。通过使用从独立实验获得的动力学参数集对上述实验依赖性进行定量描述，验证了所提出的动力学方案。这种方法可用于不同青霉素酰化酶催化的酰基转移反应的建模。
