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从人胎儿肝脏中分离、培养和鉴定具有红系、髓系、树突状细胞和自然杀伤细胞潜能的集落形成细胞。

Isolation, growth and identification of colony-forming cells with erythroid, myeloid, dendritic cell and NK-cell potential from human fetal liver.

作者信息

Muench Marcus O, Suskind David L, Bárcena Alicia

机构信息

Department of Laboratory Medicine, University of California at San Francisco. 3rd & Parnassus Ave., Room U-440; San Francisco, CA 94143-0793.

出版信息

Biol Proced Online. 2002 Jun 11;4:10-23. doi: 10.1251/bpo29.

DOI:10.1251/bpo29
PMID:12734573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC145552/
Abstract

The study of hematopoietic stem cells (HSCs) and the process by which they differentiate into committed progenitors has been hampered by the lack of in vitro clonal assays that can support erythroid, myeloid and lymphoid differentiation. We describe a method for the isolation from human fetal liver of highly purified candidate HSCs and progenitors based on the phenotypes CD38(-)CD34(++) and CD38(+)CD34(++), respectively. We also describe a method for the growth of colony-forming cells (CFCs) from these cell populations, under defined culture conditions, that supports the differentiation of erythroid, CD14/CD15(+) myeloid, CD1a(+) dendritic cell and CD56(+) NK cell lineages. Flow cytometric analyses of individual colonies demonstrate that CFCs with erythroid, myeloid and lymphoid potential are distributed among both the CD38(-) and CD38(+) populations of CD34(++) progenitors.

摘要

造血干细胞（HSCs）及其分化为定向祖细胞过程的研究，一直因缺乏能够支持红系、髓系和淋巴系分化的体外克隆分析方法而受到阻碍。我们描述了一种分别基于CD38(-)CD34(++)和CD38(+)CD34(++)表型，从人胎肝中分离高度纯化的候选造血干细胞和祖细胞的方法。我们还描述了一种在特定培养条件下，从这些细胞群体中培养集落形成细胞（CFCs）的方法，该方法支持红系、CD14/CD15(+)髓系、CD1a(+)树突状细胞和CD56(+)自然杀伤细胞系的分化。对单个集落的流式细胞术分析表明，具有红系、髓系和淋巴系分化潜能的集落形成细胞分布在CD34(++)祖细胞的CD38(-)和CD38(+)群体中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/0f15484ef297/m29f4lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/62fc8ab7c937/m29f1lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/a133a9a20f80/m29f2lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/802c76aa4c14/m29f3lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/0f15484ef297/m29f4lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/62fc8ab7c937/m29f1lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/a133a9a20f80/m29f2lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/802c76aa4c14/m29f3lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e09/145552/0f15484ef297/m29f4lg.jpg

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