Van Criekinge Wim, Beyaert Rudi
DEVGEN NV. Technologiepark 9, 9052 Zwijnaarde-Gent. Belgium.; Department of Molecular Biology. Flanders Interuniversity Institute for Biotechnology and University of Ghent, B-9000, Ghent. Belgium.
Biol Proced Online. 1999 Oct 4;2:1-38. doi: 10.1251/bpo16.
Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination.
基因组计划已接近完成，序列数据库正趋于饱和。本文讨论了双杂交系统作为一种假设生成工具的作用。除了这种相当详尽、耗费资金和人力的程序外，还可以进行更精细的功能研究。实际上，通过构建双杂交系统的杂交体，可以开发定制方法来解决特定的功能相关问题。例如，可以通过在三杂交体系中结合正向和反向方法来建立“差异”筛选。另一个非常有趣的项目是在双杂交方法中使用肽库。这可以识别出特异性极高、堪比“真正”抗体的肽。就现有技术而言，唯一的限制就是想象力。
