Tirado-Miranda María, Schmitt Artur, Callejas-Fernández José, Fernández-Barbero Antonio
Department of Physics, University of Extremadura, 10071 Cáceres, Spain.
Eur Biophys J. 2003 May;32(2):128-36. doi: 10.1007/s00249-002-0275-6. Epub 2003 Feb 15.
The different mechanisms involved in the aggregation of spherical latex particles coated with bovine serum albumin (BSA) have been studied using static and dynamic light scattering. These techniques assess the fractal dimension of the aggregates and their mean hydrodynamic radius. Particles with different degrees of surface coverage have been prepared. The net charge of the covered particles has been modified by varying the pH of the aqueous phase. The aggregation rate was measured and used to determine the importance of the different aggregation mechanisms that are responsible for these types of flocculation processes. At low and intermediate degrees of surface coverage, bridging flocculation is the principal aggregation mechanism irrespective of the electrical state of the protein-particle complexes. At high degree of surface coverage, however, weak flocculation is important only when the BSA molecules are at their isoelectric point.
利用静态和动态光散射技术研究了涂有牛血清白蛋白（BSA）的球形乳胶颗粒聚集过程中涉及的不同机制。这些技术评估了聚集体的分形维数及其平均流体动力学半径。制备了具有不同表面覆盖度的颗粒。通过改变水相的pH值来改变被覆盖颗粒的净电荷。测量了聚集速率，并用于确定导致这些絮凝过程的不同聚集机制的重要性。在低和中等表面覆盖度下，无论蛋白质-颗粒复合物的电状态如何，桥连絮凝都是主要的聚集机制。然而，在高表面覆盖度下，只有当BSA分子处于其等电点时，弱絮凝才很重要。
