Nita L E, Chiriac A P, Bercea M, Asandulesa M, Wolf Bernhard A
"Petru Poni" Institute of Macromolecular Chemistry, 41-A Grigore Ghica Voda Alley, RO-700487 Iasi, Romania.
"Petru Poni" Institute of Macromolecular Chemistry, 41-A Grigore Ghica Voda Alley, RO-700487 Iasi, Romania.
Int J Biol Macromol. 2017 Feb;95:412-420. doi: 10.1016/j.ijbiomac.2016.11.080. Epub 2016 Nov 23.
Macromolecular co-assemblies built up in aqueous solutions, by using a linear polypeptide, poly(aspartic acid) (PAS), and a globular protein, bovine serum albumin (BSA), have been studied. The main interest was to identify the optimum conditions for an interpenetrated complex formation in order to design materials suitable for biomedical applications, such as drug delivery systems. BSA surface possesses several amino- and carboxylic groups available for covalent modification, and/or bioactive substances attachment. In the present study, mixtures between PAS and BSA were investigated at 37°C in dilute aqueous solution by viscometry, dynamic light scattering and zeta potential determination, as well as in solid state by AFM microscopy and dielectric spectroscopy. The experimental data have shown that the interpolymer complex formation occurs for a PAS/BSA molar ratio around 0.541.
人们已经对通过使用线性多肽聚天冬氨酸（PAS）和球状蛋白牛血清白蛋白（BSA）在水溶液中构建的大分子共组装体进行了研究。主要目的是确定形成互穿复合物的最佳条件，以便设计适用于生物医学应用（如药物递送系统）的材料。BSA表面有几个可用于共价修饰和/或连接生物活性物质的氨基和羧基。在本研究中，通过粘度测定、动态光散射和zeta电位测定，在37°C下于稀水溶液中研究了PAS和BSA之间的混合物，还通过原子力显微镜和介电谱在固态下进行了研究。实验数据表明，当PAS/BSA摩尔比约为0.541时会形成聚合物间复合物。
