巴西牛微小双股RNA病毒的遗传多样性
Genetic diversity of bovine Picobirnavirus, Brazil.
作者信息
Navarro Juliana de Oliveira, Candido Marcelo, de Almeida-Queiroz Sabrina Ribeiro, Buzinaro Maria da Glória, Livonesi Márcia Cristina, Fernandes Andrezza Maria, de Sousa Ricardo Luiz Moro
机构信息
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenue Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil.
Department of Preventive Veterinary Medicine and Animal Reproduction, São Paulo State University (UNESP), Access Route Prof. Paulo Donato Castellani, rural, Jaboticabal, São Paulo, 14884-900, Brazil.
出版信息
Virus Genes. 2018 Oct;54(5):724-728. doi: 10.1007/s11262-018-1586-8. Epub 2018 Jul 9.
Picobirnaviruses (PBVs) are emerging and opportunistic viruses with possible zoonotic potential. In this study, we present the detection, molecular characterization, and genotypic differentiation of PBVs from genogroup I in bovine stool samples from different Brazilian regions. A high proportion of PCR-positive samples (23.4%) was detected in a total of 77 analyzed. Nucleotide identity, alignment, and phylogenetic analyses revealed high diversity among the studied sequences. The results obtained indicate, for the first time, the circulation of bovine PBVs belonging to genogroup I in different Brazilian states, with heterogeneous phylogenetic-clustering profiles.
微小双股RNA病毒（PBVs）是具有潜在人畜共患病潜力的新兴机会性病毒。在本研究中，我们展示了来自巴西不同地区牛粪便样本中I基因组PBVs的检测、分子特征及基因型分化。在总共77份分析样本中检测到高比例（23.4%）的PCR阳性样本。核苷酸同一性、比对及系统发育分析显示，所研究序列间存在高度多样性。所获结果首次表明，属于I基因组的牛PBVs在巴西不同州传播，具有异质的系统发育聚类图谱。
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