Hyun Myung Ho, Han Sang Cheol, Whangbo Sung Hee
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Kuemjeong-Ku, Pusan 609-735, South Korea.
J Chromatogr A. 2003 Apr 11;992(1-2):47-56. doi: 10.1016/s0021-9673(03)00276-0.
A new ligand exchange chiral stationary phase (CSP) has been developed by covalently bonding (R)-N,N-carboxymethyl undecyl phenylglycinol mono-sodium salt onto silica gel and applied in the resolution of alpha- and beta-amino acids. In the resolution of alpha-amino acids, the new CSP was better insome cases than the old one, which was previously developed by covalently bonding (S)-N,N-carboxymethyl undecyl leucinol mono-sodium salt onto silica gel, but worse in some other cases than the old one in terms of the separation factors (alpha). However, the new CSP wasalways much better than the old one in terms of the resolution factors (Rs). In the resolution of beta-amino acids, the new CSP was always much better than the old one in terms of both the separation and resolution factors. In an effort to characterize the new CSP, the chromatographic behaviors for the resolution of selected alpha- and beta-amino acids were investigated with the variation of the content of organic modifier and Cu(II) concentration in aqueous mobile phase and the column temperature.
通过将（R）-N，N-羧甲基十一烷基苯基甘氨醇单钠盐共价键合到硅胶上，开发了一种新型配体交换手性固定相（CSP），并将其应用于α-氨基酸和β-氨基酸的拆分。在α-氨基酸的拆分中，新的CSP在某些情况下比之前开发的旧CSP更好，旧的CSP是通过将（S）-N，N-羧甲基十一烷基亮氨醇单钠盐共价键合到硅胶上制成的，但在其他一些情况下，就分离因子（α）而言比旧的CSP更差。然而，就分离度因子（Rs）而言，新的CSP总是比旧的CSP好得多。在β-氨基酸的拆分中，就分离因子和分离度因子而言，新的CSP总是比旧的CSP好得多。为了表征新的CSP，研究了在水相流动相中有机改性剂含量、Cu（II）浓度和柱温变化时，选定的α-氨基酸和β-氨基酸拆分的色谱行为。
