Hyun Myung Ho, Han Sang Cheol, Lee Cheong Wha, Lee Yun Kyeong
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, South Korea.
J Chromatogr A. 2002 Mar 15;950(1-2):55-63. doi: 10.1016/s0021-9673(02)00035-3.
A new liquid chromatographic ligand exchange CSP has been prepared by covalently bonding (S)-N,N-carboxymethyl undecyl leucinol monosodium salt onto silica gel and employed in resolving various alpha-amino acids. The new CSP was quite good in resolving various a-amino acids and the resolution results were dependent on the type and content of organic modifier in the mobile phase. From these results, a chiral recognition model using a lipophilic interaction between the tethering alkyl group of the CSP and the substituent at the chiral center of alpha-amino acids was proposed. The liquid chromatographic resolution of alpha-amino acids on the new CSP was also found to be dependent on the Cu(II) concentration in the mobile phase and the column temperature.
一种新型液相色谱配体交换固定相已通过将（S）-N，N-羧甲基十一烷基亮氨醇单钠盐共价键合到硅胶上制备而成，并用于拆分各种α-氨基酸。这种新型固定相在拆分各种α-氨基酸方面表现出色，拆分结果取决于流动相中有机改性剂的类型和含量。基于这些结果，提出了一种手性识别模型，该模型利用固定相的连接烷基与α-氨基酸手性中心的取代基之间的亲脂相互作用。还发现，α-氨基酸在新型固定相上的液相色谱拆分取决于流动相中的Cu（II）浓度和柱温。
