Wang Xiaoyan, Wang Qiang, Chen Yi, Han Huiwan
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, PO Box 2709, Beijing 100080, China.
J Chromatogr A. 2003 Apr 11;992(1-2):181-91. doi: 10.1016/s0021-9673(03)00273-5.
A method based on pre-capillary derivatization with luminol (3-aminophthalhydrazide) for carbohydrate analysis using capillary electrophoresis with on-line chemiluminescence (CL) detection was developed. The derivatives of seven monosaccharides were separated and detected by using 200 mM borate buffer containing 100 mM hydrogen peroxide at pH 10.0 as separation electrolyte and 25 mM hexacyanoferrate in 3 M sodium hydroxide solution as post-capillary chemiluminescence reagent with separation efficiencies ranging from 160,000 to 231,000 plates per metre. The minimum amount of carbohydrate derivatized was 2 pmol (corresponding to the concentration of 2 microM). The method also provided a linear response for glucose in the concentration range of 0.1-250 microM with a mass detection limit of 420 amol or a concentration detection limit of 0.1 microM. Preliminary work using the CE-CL format to determine glucose in a rat brain microdialysis sample is presented as a typical case.
开发了一种基于鲁米诺（3-氨基邻苯二甲酰肼）预毛细管衍生化的方法，用于采用毛细管电泳-在线化学发光（CL）检测的碳水化合物分析。以pH 10.0的含100 mM过氧化氢的200 mM硼酸盐缓冲液为分离电解质，以3 M氢氧化钠溶液中的25 mM铁氰化钾为毛细管后化学发光试剂，分离并检测了七种单糖的衍生物，分离效率为每米160,000至231,000理论塔板数。衍生化的碳水化合物的最小量为2 pmol（对应于2 microM的浓度）。该方法还在0.1-250 microM的浓度范围内对葡萄糖提供线性响应，质量检测限为420 amol或浓度检测限为0.1 microM。作为典型案例，展示了使用CE-CL形式测定大鼠脑微透析样品中葡萄糖的初步工作。
