Department of Chemistry, East China Normal University, Shanghai 200062, People's Republic of China.
Talanta. 2012 May 15;93:428-32. doi: 10.1016/j.talanta.2012.02.040. Epub 2012 Feb 22.
As a kinetic process, chemiluminescence (CL) met great challenges while it was used in the detection methods coupled with capillary electrophoresis (CE). In this investigation, a newly recorded ultra-fast CL reaction of luminol-KIO(4)-K(3)Fe(CN)(6) was observed to be completed in 0.65 s. It was adopted in a simple CE-CL system efficiently to avoid the peak-tailing and overlapping. With this compact system, an indirect determination of rhamnose, d-fructose, sucrose and β-cyclodextrin was realized based on the corresponding negative CL peaks. These peaks were due to the displacement of luminol anions by the analyzed saccharide molecules in alkaline separation electrolyte. In this way, these four saccharides could be separated and determined in 16 min with adequate sensitivities and stabilities. No derivatization or pretreatment was required for the analysis, and it presents an attractive opportunity for routine tests of mono-, di- and oligo-saccharides in a compact CE-CL system, even as a microchip device.
作为一个动力学过程，化学发光（CL）在与毛细管电泳（CE）偶联的检测方法中遇到了很大的挑战。在这项研究中，观察到新记录的超快速 CL 反应，即鲁米诺-KIO(4)-K(3)Fe(CN)(6)在 0.65 秒内完成。该反应被有效地应用于一个简单的 CE-CL 系统中，以避免峰尾和重叠。使用这个紧凑的系统，基于相应的负 CL 峰，可以实现鼠李糖、d-果糖、蔗糖和β-环糊精的间接测定。这些峰是由于分析的糖分子在碱性分离电解质中取代了鲁米诺阴离子。通过这种方式，这四种糖可以在 16 分钟内分离和测定，具有足够的灵敏度和稳定性。分析不需要衍生化或预处理，为在紧凑的 CE-CL 系统中，甚至是微芯片设备中对单糖、二糖和寡糖进行常规测试提供了一个有吸引力的机会。
