Jeichitra V, Kandasamy N, Panneerselvam S
Department of Animal Genetics, Veterinary College and Research Institute, Namakkal 637 001, Tamil Nadu, India.
Trop Anim Health Prod. 2003 Apr;35(2):147-53. doi: 10.1023/a:1022825518939.
This paper reports the milk protein polymorphism, the allele frequencies of variants and the possible linkages among various combinations of milk protein phenotypes in the Kangayam cattle of south India. Milk samples from 156 Kangayam cows were typed by starch gel and polyacrylamide gel electrophoresis for caseins and whey proteins, respectively. All the four milk protein components studied, alpha(s)1-casein. beta-casein, beta-lactoglobulin and alpha-lactalbumin, exhibited polymorphism with high allele frequencies of 0.9231 +/- 0.0151 for alpha(s)1-casein C, 0.9263 +/- 0.0148 for beta-casein A, 0.9135 +/- 0.0159 for beta-lactoglobulin B and a relatively high frequency of 0.6218 +/- 0.0275 for alpha-lactalbumin A. The mean heterozygosity estimated over all the four milk protein loci was 0.2420. Genetic equilibrium was observed among all the loci studied, except alpha-lactalbumin. Linkage analysis confirmed the non-independence between alpha(s)1- and beta-caseins and between caseins and alpha-lactalbumin phenotypes.
本文报道了印度南部康盖亚姆牛的乳蛋白多态性、变异体的等位基因频率以及乳蛋白表型各种组合之间可能的连锁关系。分别通过淀粉凝胶电泳和聚丙烯酰胺凝胶电泳对156头康盖亚姆奶牛的乳样进行酪蛋白和乳清蛋白分型。所研究的所有四种乳蛋白成分，即α(s)1-酪蛋白、β-酪蛋白、β-乳球蛋白和α-乳白蛋白，均表现出多态性，α(s)1-酪蛋白C的等位基因频率较高，为0.9231±0.0151，β-酪蛋白A为0.9263±0.0148，β-乳球蛋白B为0.9135±0.0159，α-乳白蛋白A的频率相对较高，为0.6218±0.0275。在所有四个乳蛋白位点上估计的平均杂合度为0.2420。在所研究的所有位点中，除α-乳白蛋白外，均观察到遗传平衡。连锁分析证实了α(s)1-酪蛋白和β-酪蛋白之间以及酪蛋白和α-乳白蛋白表型之间的非独立性。
