Deschenes Michael R, Will Kristin M, Booth Frank W, Gordon Scott E
Department of Kinesiology, The College of William and Mary, Williamsburg, VA 23187-8795, USA.
J Neurol Sci. 2003 Jun 15;210(1-2):5-10. doi: 10.1016/s0022-510x(02)00455-0.
This study assessed the effect of muscle unloading on the neuromuscular system. Sixteen male Fischer 344 rats were randomly assigned to either a hindlimb suspension (unloaded) or control group (N=8/group) for 16 days. Following this intervention period, pre- and postsynaptic features of the neuromuscular junctions (NMJs) of soleus muscles were stained with cytofluorescent techniques, and myofibers were histochemically stained for ATPase activity. The data indicate that 16 days of muscle unloading resulted in significant (P<0.05) atrophy among myofibers (>50%) that was evident among all three major fiber types (I, IIA and IIX), but failed to significantly alter any aspect of NMJ morphology quantified. These results demonstrate an impressive degree of NMJ resilience despite dramatic remodeling of associated myofibers. This may be of benefit during post-unloading rehabilitative measures where effective neuromuscular communication is essential.
本研究评估了肌肉卸载对神经肌肉系统的影响。将16只雄性Fischer 344大鼠随机分为后肢悬吊（卸载）组或对照组（每组n = 8），为期16天。在此干预期后，采用细胞荧光技术对比目鱼肌神经肌肉接头（NMJ）的突触前和突触后特征进行染色，并用组织化学方法对肌纤维进行ATP酶活性染色。数据表明，16天的肌肉卸载导致所有三种主要纤维类型（I、IIA和IIX）的肌纤维出现显著（P<0.05）萎缩（>50%），但未能显著改变所量化的NMJ形态的任何方面。这些结果表明，尽管相关肌纤维发生了显著重塑，但NMJ具有令人印象深刻的恢复力。这在卸载后康复措施中可能有益，因为有效的神经肌肉通讯至关重要。
