胆碱酯酶在大鼠膀胱收缩性中的作用。

The role of cholinesterases in rat urinary bladder contractility.

作者信息

Nakahara Tsutomu, Kubota Yuko, Sakamoto Kenji, Ishii Kunio

机构信息

Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.

出版信息

Urol Res. 2003 Jul;31(3):223-6. doi: 10.1007/s00240-003-0326-1. Epub 2003 May 8.

DOI:10.1007/s00240-003-0326-1

PMID:12736766

Abstract

This study examines the effects of inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) on acetylcholine (ACh)-induced contraction in rat urinary bladder smooth muscle. Neostigmine, a non-selective ChE inhibitor, caused concentration-dependent contractions in rat urinary bladder strips, whereas tetraisopropylpyrophosphoramide (iso-OMPA; a BuChE inhibitor) failed to affect the resting tone of the preparations. Neostigmine (1 microM) markedly augmented the contractile responses to ACh. Although iso-OMPA (10 microM) also potentiated ACh-induced contraction, the effect was less than that evoked by neostigmine. The activities of AChE in rat urinary bladder strips were significantly (P<0.05) higher than those of BuChE. These results indicated that AChE, rather than BuChE, plays an important role in controlling ACh-induced contractions of rat urinary bladder.

摘要

本研究考察了抑制乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）对大鼠膀胱平滑肌中乙酰胆碱（ACh）诱导收缩的影响。新斯的明，一种非选择性胆碱酯酶抑制剂，可引起大鼠膀胱条带浓度依赖性收缩，而四异丙基焦磷酰胺（iso - OMPA；一种BuChE抑制剂）未能影响标本的静息张力。新斯的明（1微摩尔）显著增强了对ACh的收缩反应。虽然iso - OMPA（10微摩尔）也增强了ACh诱导的收缩，但作用小于新斯的明所引起的作用。大鼠膀胱条带中AChE的活性显著（P<0.05）高于BuChE的活性。这些结果表明，在控制大鼠膀胱ACh诱导的收缩中，起重要作用的是AChE而非BuChE。